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Isolation, Screening and Optimized Production of Extracellular Xylanase under Submerged Condition from Aspergillus Flavus Mtcc 9390

Bharat Bhushan
2012 Enzyme Engineering  

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In order to isolate xylanolytic microbial strains, screening and isolation was done using agricultural waste and decaying biomass. The enzyme super-secreter Aspergillus flavus MTCC 9390 was selected for optimized production of xylanase. Various process variables were optimized using conventional 'one-variable-at-a-time' approach which involves varying a single independent variable and maintaining others at a constant level. All culture conditional variables had profound influence on enzyme
more » ... ction and 15-30% increase was brought by nitrogen source only. A synergistic five-fold increase in xylanase production was achieved when an inoculums size of 2 x 10 6 spores/ mL was incubated in modified Czapek Dox-A for 6 days at pH 6.0 and temperature 45ºC under static conditions in submerged fermentation.
doi:10.4172/eeg.1000103 fatcat:z67rpsumabhd5ahowwpntybvtm
Citation

Bharat Bhushan. "Isolation, Screening and Optimized Production of Extracellular Xylanase under Submerged Condition from Aspergillus Flavus Mtcc 9390." Enzyme Engineering 01.02 (2012)