Analysis of Extracellular Matrix Proteins in Malignant Chicken Cell Lines

Takumi KOYAMA, Yasuyuki NAKAJIMA, Katsuhiro MIURA, Mika YAMAZAKI, Mika SHINOZAKI, Tatsuko KUMAGAI, Misao SAKANIWA
1997 Journal of Veterinary Medical Science  
The adherent chicken cell line, MDCC-MSB1-41C, was highly transplantable and metastatic in vivo, compared with the parental non-adherent cell line MDCC-MSB1 from Marek's disease (MD) lymphoblastoid tumor. For clarification of differences in extracellular matrix proteins in MSB1-41C and MSB1 cells, examination was made of various components of extracellular matrix proteins. A detachment experiment indicated the protein(s) recognizing the Arg-Gly-Asp (RGD) sequence, the minimum structure required
more » ... structure required for recognition by the cell-surface receptors, is essential for the adherent character. Immunoblot assay using antibodies showed increased expression of fibronectin, fibronectin receptors, and vinculin on MSB1-41C cell lines. RGD-directed integrins mediate important cell-cell adhesive interaction and these interactions with extracellular matrix proteins may thus possibly be requisite for migration, proliferation and metastatic dissemination of MDCC-MSB1-41C cells. The RGD-containing peptide in the culture medium could cause detachment of cultured adhesive lymphoid leukosis LSCC-1104X5 cells from the dish too. -KEY WORDS: chicken cell line, fibronectin, vinculin.
doi:10.1292/jvms.59.405 pmid:9192366 fatcat:u6t6fg6wybb2njzl67ixypbk7e