Isolation of a Bacterium Possessing a Haloacid Dehalogenase from a Marine Sediment Core

Yoko Chiba, Takashi Yoshida, Norihiro Ito, Hiroshi Nishimura, Chiaki Imada, Hisato Yasuda, Yoshihiko Sako
2009 Microbes and Environments  
A monobromoacetic acid-resistant bacterium, Bacillus strain I37c, was isolated from a marine sediment core. The strain grew in a medium containing 1.8 mg mL −1 of monobromoacetic acid. It produced constitutively a 2-haloacid dehalogenase that catalyzed the dehalogenation of monobromoacetic acid, monochloroacetic acid, and both L-and D-2-chloropropionic acid. The optimal pH and temperature for the activity measured using a partly purified enzyme were similar to those of known group I haloacid dehalogenases.
doi:10.1264/jsme2.me09123 pmid:21566385 fatcat:d562mlqlgrejdo4canedfpa3py