Micronucleus assay in genotoxicity assessment

Viera Schwarzbacherova, Katarína Šiviková, Beáta Holečková, Ján Dianovský
2016 Acta Fytotechnica et Zootechnica  
Three different pesticide formulations were tested for micronuclei production and cell cycle arrest. Micronucleus test represents a suitable method for assessing chromosome damage because both chromosome breakage (clastogenicity) and chromosome loss (aneugenicity) can be measured simultaneously. In the experiments for 24 and/or 48 h exposure, no significant increase (P < 0.05) in MN frequency was found in comparison with negative control. On the contrary, the values of CBPI (cytochalasin
more » ... cytochalasin blocked proliferative index) were significantly reduced in a dose dependent manner (P < 0.05, P < 0.01, P < 0.001) for both exposure time. Our results indicated an expressive cytotoxic effect of various pesticides in cultivated bovine peripheral lymphocytes.
doi:10.15414/afz.2016.19.si.93-95 fatcat:tqmys2o245bq3o43gzmh75d77q