Changes in Gene Expression during Larval Development of Calliphora vicina Induced by Ecdysterone

Peter Mascheck, Klaus Scheller, Peter Karlson
1977 Zeitschrift für Naturforschung C - A Journal of Biosciences  
The influence of the insect hormone ecdysterone on the composition of the RNA from the fat bodies of Calliphora larvae was investigated by hybridization experiments. DNA was iodinated in vitro with 125J. In saturation experiments, total RNA was hybridized to total DNA; under similar conditions, poly (A) RNA was hybridized to unique DNA sequences. RNA isolated from animals treated with ecdysterone exhibits a higher percentage of hybridization than a corresponding control RNA from the same
more » ... rom the same developmental stage, a result even more prominent when poly (A) RNA was hybridized with unique DNA. It is concluded that ecdysterone induces the transcription of new unique DNA sequences.
doi:10.1515/znc-1977-5-620 fatcat:3c2tjr72nngl7oj6phmbjtsgl4