VALIDATED ZERO AND FIRST ORDER DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF POORLY SOLUBLE ANTIRETROVIRALS

Himaja Malipeddi, Kalpana Jayapalu, Chaitra Muralidhara
2015 International Journal of Research in Ayurveda and Pharmacy  
Two highly simple, sensitive and rapid derivative spectrophotometric methods were developed for the estimation of efavirenz and nevirapine in bulk and pharmaceutical dosage forms. The standard and sample solutions were prepared using methanol and distilled water in the ratio of 40:60 (azeotropic mixture). The spectrophotometric estimation of efavirenz and nevirapine was carried out using the using the zero order derivative values measured at 247 and 282 nm respectively and the first order
more » ... e first order derivative values measured at 257 and 299 nm respectively. Calibration graphs constructed at their wavelengths of determination were linear within the concentration range of 4-24 μg/ml for zero order and first order derivative spectrophotometric method. Both the proposed methods have been extensively validated as per ICH guidelines. No significant difference between the performance of the proposed methods regarding the mean values and standard deviations were noticed and is suitable for the routine quality control application of active drugs in pharmaceutical formulations.
doi:10.7897/2277-4343.06371 fatcat:sisnimou5va5rksazqsnlx3bgi