In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria

Ariana Fonseca, Fernanda Estrela, Thaís Moraes, Luiza Carneiro, Jairo Bastos, Raquel Santos, Sérgio Ambrósio, Carlos Martins, Rodrigo Veneziani
2013 Molecules  
We evaluated the antibacterial activity of three diterpenes isolated from natural sources against a panel of microorganisms responsible for bovine mastitis. ent-Copalic acid (CA) was the most active metabolite, with promising MIC values (from 1.56 to 6.25 µg mL −1 ) against Staphylococcus aureus (ATCC and clinical isolate), Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus dysgalactiae. We conducted time-kill assays of CA against S. aureus, a commensal organism considered
more » ... rganism considered to be a ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. In the first 12 h, CA only inhibited the growth of the inoculums (bacteriostatic effect), but its bactericidal effect was clearly noted thereafter (between 12 and 24 h). In conclusion, CA should be considered for the control of several Gram-positive bacteria related to bovine mastitis.
doi:10.3390/molecules18077865 pmid:23884123 fatcat:txqpcblvbfgpjoadjgywm2ejgy