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Footprinting is a valuable tool for studying DNA-protein contacts. However, it usually involves expensive, tedious and hazardous steps such as radioactive labeling and analyses on polyacrylamide sequencing gels. We have developed an easy four-step footprinting method involving( i ) the generation and purification of a PCR fragment that is fluorescently labeled at one end with 6-carboxyfluorescein; ( ii ) brief exposure of the fragment to a DNA-binding protein and then DNase I; ( iii )doi:10.2144/00295st05 pmid:11084866 fatcat:qqep4f5ex5cqpluealla655pne