Effects of Dexamethasone and Electrical Stimulation Treatment on Skeletal Muscle Cell Derived Exosome's MicroRNA and Muscle Atrophy and Hypertrophy Related Proteins Expression

김정석, Yoon,Chung-Su, 박대령, 박소영
2014 Exercise Science  
The purpose of this study was to examine the effect of dexamethasone (Dex) treatment and electrical stimulation (ES) on skeletal muscle cell derived exosome's microRNA (miR) profile and its effect on skeletal muscle signal protein expression. Exosome obtained from two different C2C12 cell were treated with Dex or ES. Then we profiled exosome's miR expression and atrophy related miR-1, miR-206, miR-322 were significantly increased with DEX however, hypertrophy related miR-23a was significantly
more » ... was significantly increased only in the ES. Further we treated two different types of exosome to Dex treated skeletal muscle primary cell and two types of exosome differently regulated atrophy related protein (FOXO/MurF) and hypertrophy related protein (mTOR/p70S6K) phosphorylaton. In conclusion, the results indicate that atrophy or hypertrophy signal induces different exosome including different profile of miR and it plays important role in skeletal muscle atrophy and hypertrophy.
doi:10.15857/ksep.2014.23.4.347 fatcat:aeq44cg65vex3c56o5zuurx6na