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For quantification of histamine, we developed a highly specific and sensitive method of postcolumn fluorescence detection HPLC using a tetrahydrofuran solution of orthophthalaldehyde. Its detection limit was 2 -5 pg. This method was applied to the measurement of histamine in human blood and urine samples. Analysis by LC/MS and NMR suggested that the fluorophore in this method has an isoindole structure.doi:10.2116/analsci.10.259 fatcat:2di3whncbnaypkjty4c2acqmj4