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The Plant Cell
To study the role of initiation codon context in chloroplast protein synthesis, we mutated the three nucleotides immediately upstream of the initiation codon (the ؊ 1 triplet) of two chloroplast genes in the alga Chlamydomonas reinhardtii . In prokaryotes, the ؊ 1 triplet has been proposed to base pair with either the 530 loop of 16S rRNA or the extended anticodon of fMet-tRNA. We found that in vivo, none of the chloroplast mutations affected mRNA stability. However, certain mutations did causedoi:10.2307/3871514 pmid:11595808 fatcat:rjv6asb5h5gfdbptz4ycqnvbqu