Antimicrobial screening and GC-MS analysis of bioactive compounds from strains of Pseudomonas aeruginosa isolated from poultry fecal littered soil in Ibadan, Nigeria [article]

Olumuyiwa S. Alabi, Oluwasegun B. Koleoso, Akedele M. Abiala
2020 Figshare  
ABSTRACTThe emergence of pathogens exhibiting multiple antibiotic resistance (MDR) is bringing a thwart to available chemo-therapeutic agents thus the urgency in the search for bioactive agents that will be effective against the MDR pathogens. This study screened isolates of Pseudomonas aeruginosa from poultry fecal littered soil in two selected locations in northern Ibadan, southwest Nigeria (University of Ibadan Teaching Research Farm and Ayede community) for production of bioactive
more » ... bioactive compounds, evaluated their activities against selected MDR bacteria pathogens and analyzed the crude ethylacetate extract using GC-MS. A total of 178 strains of P. aeruginosa were isolated on cetrimide agar and identified presumptively by standard biochemical test. Primary and secondary screening of the whole cell and cell free supernatant ethylacetate extracts were done by perpendicular streaking and agar-well diffusion methods respectively against selected clinical MDR bacteria pathogens. Five out of the 178 strains of the presumptive P. aeruginosa with excellent antibacterial activity were further confirmed by 16S rRNA amplification and sequencing. Cell free super-natant ethylacetate extracts of two most active strains among the five (denoted with code APC2-1 and APC2-14) were subjected to GC-MS analysis of the metabolites. The five P. aeruginosa strains were identified as P. aeruginosa MR24 strains 1, 2, 3, Pseudomonas sp SD12 and P. aeruginosa Kasamber 1 which were closely related phylo-genetically. The bioactive compounds produced by the isolates that were studied had greater activity on Gram positive than Gram negative pathogens. GC-MS analysis revealed a total of 19 different compounds (1-hepta-decanamine; 2-ethyl-1-hexanamine; 3-butyn-1-ol; Pent-3-enylamine; Pent-4-enylamine; Propane; Acetaldehyde; Ethyleneoxide; hydroxyacetonitrile; Bromo-acetonitrile; Azetidine; 2-methyl-Azetidine; Aminocaproic acid; 18-nonadecen-1-amine; 5-aminovaleric acid; 2,2-dimethyl-1,3-pr [...]
doi:10.6084/m9.figshare.12278864.v1 fatcat:krdu6tjxcnei3bk4q6vlmb6mvm