Isolation, Identification, and Characterization of a Keratin-degrading Bacterium Chryseobacterium sp. P1-3

Sung-Jun Hong, Gun-Seok Park, Byung Kwon Jung, Abdur Rahim Khan, Yeong-Jun Park, Chang-Hyun Lee, Jae-Ho Shin
2015 Journal of Applied Biological Chemistry  
In this study, a keratin-degrading bacterium was isolated from soil contaminated with feather waste. The isolated strain was identified as Chryseobacterium sp. P1-3 on the basis of the 16S rRNA gene sequence alignment. Chryseobacterium sp. P1-3 is currently used in various biotechnological applications (e.g., in the hydrolysis of poultry feathers). It hydrolyzed the feather meal within 2 days and possesses a high level of keratinase activity (98 U/mL). The keratinase, partially purified from
more » ... s strain, prefers casein as a substrate and shows optimal activity at a temperature of 30 o C and at a pH of 8.0. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons. org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
doi:10.3839/jabc.2015.039 fatcat:yjyf6gfzyvbvjilfwhhvx5zzsm