MOESM1 of Testing a MultiTEP-based combination vaccine to reduce Aβ and tau pathology in Tau22/5xFAD bigenic mice

Hayk Davtyan, Armine Hovakimyan, Sepideh Kiani Shabestari, Tatevik Antonyan, Morgan Coburn, Karen Zagorski, Gor Chailyan, Irina Petrushina, Olga Svystun, Emma Danhash, Nikolai Petrovsky, David Cribbs (+3 others)
2019 Figshare  
Additional file 1: Figure S1. Humoral immune responses in female and male T5x mice vaccinated with different vaccines. Concentration of antibodies binding to Aβ42 (a, d), tau2-18 peptide (b, e) and full-length recombinant tau protein (c, f) were detected in sera by ELISA. Lines represent average. Statistically significant differences were examined using unpaired t-test (**p ≤ 0.01; Control group - n=8 for female and n=14 for male; AV-1959R/A group - n=6 for female and n=8 for male, AV-1980R/A
more » ... male, AV-1980R/A group - n=6 for female and n=8 for male and AV-1959R/A+AV-1980RA group - n=5 for female and n=7 for male). Figure S2. Effect of protein vaccination on Aβ proteins in female, T5x mice. Level of human Aβ42 (a and d), Aβ40 (b and e) and Aβ38 (c and f) peptides in brain soluble (a-c) and insoluble (d-f) extractions were analyzed by MSD assay. Error bars represent average ± SEM. Statistically significance were calculated against Control group using ANOVA test (*p ≤ 0.01; Control group - n=8 for female and n=14 for male; AV-1959R/A group - n=6 for female and n=8 for male, AV-1980R/A group - n=6 for female and n=8 for male and AV-1959R/A+AV-1980RA group - n=5 for female and n=7 for male). Figure S3. Effect of protein vaccination on Aβ proteins in male, T5x mice. Level of human Aβ42 (a and d), Aβ40 (b and e) and Aβ38 (c and f) peptides in brain soluble (a-c) and insoluble (d-f) extractions were analyzed by MSD assay. Error bars represent average ± SEM. Statistically significance were calculated against Control group using ANOVA test (*p ≤ 0.05; ***p ≤ 0.001; Control group - n=8 for female and n=14 for male; AV-1959R/A group - n=6 for female and n=8 for male, AV-1980R/A group - n=6 for female and n=8 for male and AV-1959R/A+AV-1980RA group - n=5 for female and n=7 for male). Figure S4. Effect of protein vaccination on tau proteins in female, T5x mice. Level of human total tau protein (a, f) and several phosphorylated tau species (b-e and g-j) in brain soluble (a-e) and insoluble (fj) extractions were analyzed by ELISA. Error bars rep [...]
doi:10.6084/m9.figshare.11393559.v1 fatcat:mwnglpjhbnfwtajtafne7ui6tm