Development of PCR for specific determination of root-knot nema­tode Meloidogyne incognita

B. Tesařová, M. Zouhar, P. Ryšánek
2011 Plant Protection Science  
PCR primers designed from the gene sequence for the SEC 1 oesophageal gland protein were used to specifically detect and differentiate the root-knot nematode Meloidogyne incognita from other species from the genus Meloido­gyne. Amplification products were obtained from five M. incognita populations from different origins whereas DNA from M. fallax, M. javanica, M. arenaria, M. chitwoodi and M. hapla was not amplified. DNA extracted from different materials (females, root galls and spiked
more » ... galls and spiked soil) could easily be used for M. incognita detection. One female gave sufficient amount of DNA for detection. Together with mitochondrial DNA this is one of the first attempts to use a gene outside of ITS regions for species specific PCR in the genus Meloidogyne.  
doi:10.17221/3823-pps fatcat:qorvhi2no5ambl6r6dlnnsixke