DNA methylation in sugarcane somaclonal variants assessed through methylation-sensitive amplified polymorphism

J.H.M.B. Francischini, E.L. Kemper, J.B. Costa, J.R.V. Manechini, L.R. Pinto
2017 Genetics and Molecular Research  
Micropropagation is an important tool for large-scale multiplication of plant superior genotypes. However, somaclonal variation is one of the drawbacks of this process. Changes in DNA methylation have been widely reported as one of the main causes of somaclonal variations in plants. In order to investigate the occurrence of changes in the methylation pattern of sugarcane somaclonal variants, the MSAP (methylation-sensitive amplified polymorphism) technique was applied to micro-propagated
more » ... o-propagated plantlets sampled at the third subculture phase. The mother plant, in vitro normal plantlets, and in vitro abnormal plantlets (somaclonal variants) of four sugarcane clones were screened against 16 MSAP selective primers for EcoRI/MspI and EcoRI/HpaII restriction enzymes. A total of 1005 and 1200 MSAPderived markers with polymorphism percentages of 28.36 and 40.67
doi:10.4238/gmr16029585 pmid:28481402 fatcat:q7r5ewkefbhl5a4tduokwozybi