D. Rizkiyanti, I N. Wirajana, I W. B. Suyasa
2020 Jurnal Kimia (Journal of Chemistry)  
One way to restore soil fertility exposed to pesticides such as chlorpyrifos is by bioremediation using Effective Microorganisms (EM). The purpose of this study was to determine EM microbial resistance and chlorpyrifos degrading enzyme activity in organic farming soil sample.The organic farming soil sample used previously has been exposed to EM for a long time. This soil sample was divided into two treatments, namely sterilized by autoclaving and not sterilized (thus indigenous microbes remain
more » ... us microbes remain alive). The next two soil samples were with and without addition of EM with incubation time of 3, 5, and 7 days. The results of this study indicated that EM microbes could withstand exposure to chlorpyrifos to a concentration of 56 ppm. The phenomenon of chlorpyrifos degrading enzyme activity was found in sterile soil samples with EM addition of 0.017 U/mL during 7-day incubation with a decrease in chlorpyrifos levels of 16.65%. The highest activity of chlorpyrifos degrading enzyme was found in unsterile soil sample, which amounted to 0.039 U/mL during the 3-day incubation period with a decrease in chlorpyrifos levels of 74.11%. The highest decrease in chlorpyrifos was found in unsterile soil sample by 80.04% with an incubation time of 5 days. Keywords: chlorpyrifos, bioremediation, microbial EM (Effective Microorganisms)
doi:10.24843/jchem.2020.v14.i02.p04 fatcat:q7mipn772ff3tjgzcwc2vtgmye