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Motivation Calcium imaging is quickly becoming a prominent paradigm to collect data in neuroscience. To maximally utilize the power of this technique, complementary analytical tools can be built. Goal We aim to develop analytical tools to facilitate inferring spike trains from fluorescent observations, fitting tuning curves, and inferring population connectivity, given only short sequences of possibly very noisy, low temporal resolution, and saturating fluorescence images. Solution By framingdoi:10.6084/m9.figshare.1285824.v1 fatcat:vdmywhkpcrcoxekd3wq7ioysxq