Acquisition of pig intramuscular preadipocytes through dedifferentiation of mature adipocytes and establishment of optimal induction conditions

W. Sun, H. Wang, Y. Li, X. Zhou, Y. Teng, J. Chen
2013 Genetics and Molecular Research  
Adipose-derived intramuscular preadipocytes of piglets in the cytoplasm and expression patterns of peroxisome proliferatoractivated receptor gamma 2 (PPARg2), CCAAT/enhancer binding protein alpha (C/EBPa), lipoprotein lipase (LPL), and adiponectin genes, which were all similar to those observed in previous preadipocyte studies. We optimized the induction conditions for intramuscular preadipocytes by adding 0.25 nM dexamethasone (DEX), 5 mg/mL insulin (INS), and 0.1 mM
more » ... tyl-1-methylxanthine (IBMX). Therefore, this study provides a new model for studying the mechanisms of intramuscular preadipocyte differentiation and metabolism.
doi:10.4238/2013.november.26.2 pmid:24338386 fatcat:4q34g3wdrjdu7cvucjgrjmqjcu