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A new patchoulane-type sesquiterpenoid glycoside (1), together with five known sesquiterpenoids (2–6), was isolated from the roots of Croton crassifolius. Their structures were elucidated on the basis of spectroscopic methods. This is the first report about the sesquiterpenoid glycoside from C. crassifolius. All the isolated compounds 1–6 were evaluated for their cytotoxic activities against human tumour cell lines HL-60 and A549, but none showed significant activity.doi:10.6084/m9.figshare.3851406 fatcat:n3ovu4psqzdcpmhhw4uswjoqam