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Copper(II) chlorophyll a and b, i.e. (pheophytinato a)copper(II) and (pheophytinato b)copper(II), were prepared and purified by semi-preparative reversed-phase high performance liquid chromatography (HPLC). The spectroscopic properties of these compounds, which so far have been little studied, are given in detail. Separation of copper(II) chlorophyll a and b is achieved with a C18-bonded silica column and a mobile phase of acetoneacetonitrile (50:50, v/v). A rapid and accurate HPLC method withdoi:10.2116/analsci.4.599 fatcat:b5uzswszvncd5neqlwx2vrwcqi