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Ni nanoparticles (NPs), multi-walled carbon nanotubes (MWCNT), and MWCNT/Ni were compared for the first time in enhancing power production and wastewater treatment efficiency of a two-chambered microbial fuel cell (MFC). The cathode electrode – a 3D carbon fiber brush – was modified for the first time using different amounts of the three types of nanomaterials. Closed-circuit voltage of the cell was recorded, and chemical oxygen demand (COD) of the anode solution was measured with time. Thedoi:10.6084/m9.figshare.9928856 fatcat:b4brvh3ay5dtbhxahyqsa7moc4