Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil

Cleverson Busso, Edilson Nobuyoshi Kaneshima, Francisco de Assis Franco, Carmen Boto Querol, Marialba Avezum Alves de Castro-Prado
2007 Ciência Rural  
Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR
more » ... s. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the clonal origin of F. graminearum isolates.
doi:10.1590/s0103-84782007000600050 fatcat:65iidkvgqfhz7lswmm25n7roci