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Expression and Supramolecular Assembly of Recombinant α1(VIII) and α2(VIII) Collagen Homotrimers
Journal of Biological Chemistry
Collagen VIII is an extracellular matrix macromolecule comprising two polypeptide chains, ␣1(VIII) and ␣2(VIII), that can form homotrimers in vitro and in vivo. Here, recombinant collagen VIII was expressed to study its supramolecular assembly following secretion. Cells transfected with ␣1(VIII) or ␣2(VIII) assembled and secreted homotrimers that were stable in denaturing conditions and had a molecular mass of ϳ180 kDa on SDS-PAGE gels. Co-transfection with prolyl 4-hydroxylase generateddoi:10.1074/jbc.m305805200 pmid:14990571 fatcat:c5djkmt2ubhv7clssns5ovxipq