The possibility of diabetic patients to develop various severe and fatal infections has been rising continuously. In diabetic patients urinary tract infection is very common. Due to the recurrent consumption of antibiotics and the usage of broad spectrum antibiotics, antibiotic resistant pathogens may develop in the urinary tract of diabetic patients. If untreated, UTI can lead to serious complications. Urine samples were taken from the Health Centre of Punjab University. Urine samples were

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... ed on CLED media. Then identification of microorganisms was done by using the method of gram staining and by biochemical testing while some were identified by using API kit. After this, the sensitivity and resistance pattern of these uro-pathogens against different antibiotics were also checked by disk agar diffusion method. Physical and biochemical parameters of diabetic patients were also checked. Our results suggest that the presence of surplus glucose in the urine promotes the growth of pathogens that leads to UTI in diabetic patients. Improved glycemic control in diabetics may help in controlling the UTIs. The assessment of physical and biochemical parameters will be useful in the early diagnosis and prediction of diabetes mellitus. Sensitivity testing before antibiotic therapy may also help in the treatment and to prevent the resistance of antibiotics.