Aspergillus nidulans is a well-established nonpathogenic laboratory model for the opportunistic mycopathogen, A. fumigatus . Some recent studies have focused on possible functional roles of glycosphingolipids (GSLs) in these fungi. It has been demonstrated that biosynthesis of glycosylinositol phosphorylceramides (GIPCs) is required for normal cell cycle progression and polarized growth in A. nidulans (Cheng, J., T.-S. Park, A. S. Fischl, and X. S. Ye. 2001. Mol. Cell Biol. 21: 6198-6209);

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... er, the structures of A. nidulans GIPCs were not addressed in that study, nor were the functional significance of individual structural variants and the downstream steps in their biosynthesis. To initiate such studies, acidic GSL components (designated An-2, -3, and -5) were isolated from A. nidulans and subjected to structural characterization by a combination of one-dimensional (1 -D) and 2-D NMR spectroscopy, electrospray ionizationmass spectrometry (ESI-MS), ESI-MS/collision-induced decomposition-MS (MS/CID-MS), ESI-pseudo-[CID-MS] 2 , and gas chromatography-MS methods. All three were determined to be GIPCs, with mannose as the only monosaccharide present in the headgroup glycans; An-2 and An-3 were identified as di-and trimannosyl inositol phosphorylceramides (IPCs) with the structures Man ␣ 1 → 3Man ␣ 1 → 2Ins1-P-1Cer and Man ␣ 1 → 3(Man ␣ 1 → 6)Man ␣ 1 → 2Ins1-P-1Cer, respectively (where Ins ‫؍‬ myo -inositol, P ‫؍‬ phosphodiester, and Cer ‫؍‬ ceramide). An-5 was partially characterized, and is proposed to be a pentamannosyl IPC, based on the trimannosyl core structure of An-3. -Bennion, B., C. Park, M. Fuller, R. Lindsey, M. Momany, R. Jennemann, and S. B. Levery. Glycosphingolipids of the model fungus Aspergillus nidulans : characterization of GIPCs with oligo-␣ -mannose-type glycans. J. Lipid Res. 2003. 44: 2073-2088.