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Proteins interact with other proteins, DNA, and other molecules, forming large-scale protein interaction networks and for easy analysis, clustering methods are needed. Regularized Markov clustering algorithm is an improvement of MCL where operations on expansion are replaced by new operations that update the flow distributions of each node. But to reduce the weaknesses of the RMCL optimization, Pigeon Inspired Optimization Algorithm (PIO) is used to replace the inflation parameters. Thedoi:10.24042/djm.v3i3.6822 fatcat:6vp75fu6r5chzll653vg6bsfca