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This paper presents the results of extensive field research on the occurrence of Rubus L. species in the Czech Republic, especially in the frontier areas near the German border. In this area, Rubus adornatus, R. fasciculatiformis and R. perlongus were newly discovered. The other species, R. ambulans and R. stohrii, were discovered in the eastern and northern parts of Bohemia. A description, an illustration, photos and a list of localities are provided for each species. Taxonomy anddoi:10.12657/denbio.075.014 fatcat:5jcpskrz7bh2xh32nxoplh567q