Migration of Neural Stem Cells in Hippocampal Slices in Hypoxia Modeling

Yulia Stukach, Khalil x Gainutdinov, Margarita Dosina, Svetlana Pashkevich, Viacheslav Andrianov, Andrew Denisov, Tatiana Bogodvid, Guzel Yafarova, Sergei Bushuk, Tatiana Kuznetsova, Vladimir Kulchitsky
2017 Journal of Stem Cells and Regenerative Therapy  
8 series of experiments were conducted at hippocampal slices of 4-week rats along with normobaric hypoxia modelling and change of nitrogen monoxide concentration in hippocampal tissue. Stem cells pool was found at Gyrus dentate (GD) and across slices in 90-100 sec after decapitation, elimination of brain and preparation of hippocampal slices with subsequent application at slices of FITC-conjugated monoclonal CD90 antibodies. Then hippocampal slices were incubated for 10 minutes in artificial
more » ... ebrospinal fluid, which contains 5 times less oxygen compared to recommended carbogen perfusion (95% O2 and 5% CO2). Fluorescent stem cells were found at Gyrus dentate and different parts of hippocampal slices periphery. Number of these cells in hippocampus increased in combination of hypoxia with L-arginine (nitrogen monoxide precursor) and dramatically decreased in combination with inhibitors of nitrogen monoxide synthase (L-NAME). Zygoscient Research Insights 2 JSCRT Volume-1 | Issue-1 December, 2016 Volume-1 | Issue-1 December, 2016 after the perfusion of hippocampal slices with NO-synthase inhibitor (10 micromolar, 1 ml of L-NAME) in the artificial cerebrospinal fluid saturated with 5% CO 2 and 95% O 2 .] CONCLUSION So, as a result, such in vitro technique is one of the ways of minimization of experimental animals' amount for investigational tasks solving in the process of medical substances and other factors screening to assess preliminarily their ability to affect reparative functions of stem cells [14] both independently and in combination with hypoxic factor . In summary, results of performed studies demonstrate ability and advisability of initial in vitro analysis of SC migration mechanisms for subsequent detailing of brain plastic functions in case of neurodestructive processes development in vivo [15, 16] .
doi:10.28967/jscrt.2016.01.16002 fatcat:kbnakg5zgzbbvmaq5cmdkx7g3m