Reconstruction of Bacterial and Viral Genomes from Multiple Metagenomes

Ankit Gupta, Sanjiv Kumar, Vishnu P. K. Prasoodanan, K. Harish, Ashok K. Sharma, Vineet K. Sharma
2016 Frontiers in Microbiology  
Several metagenomic projects have been accomplished or are in progress. However, in most cases, it is not feasible to generate complete genomic assemblies of species from the metagenomic sequencing of a complex environment. Only a few studies have reported the reconstruction of bacterial genomes from complex metagenomes. In this work, Binning-Assembly approach has been proposed and demonstrated for the reconstruction of bacterial and viral genomes from 72 human gut metagenomic datasets. A total
more » ... 1156 bacterial genomes belonging to 219 bacterial families and, 279 viral genomes belonging to 84 viral families could be identified. More than 80% complete draft genome sequences could be reconstructed for a total of 126 bacterial and 11 viral genomes. Selected draft assembled genomes could be validated with 99.8% accuracy using their ORFs. The study provides useful information on the assembly expected for a species given its number of reads and abundance. This approach along with spiking was also demonstrated to be useful in improving the draft assembly of a bacterial genome. The Binning-Assembly approach can be successfully used to reconstruct bacterial and viral genomes from multiple metagenomic datasets obtained from similar environments.
doi:10.3389/fmicb.2016.00469 pmid:27148174 pmcid:PMC4828583 fatcat:5fxi53qbobcnlaxfbgd5tza2h4