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We observed optical sum frequency generation (SFG) images of cross-sections of glutinous rice grains, in order to test a possibility of the SFG microscopy as a tool for monitoring polysaccharide species in rice grains. The SFG response in the CH vibration range was the most intense in the crush cell layer at the edge of the endosperm adjacent to the embryo probably due to optical reflection and scattering effect by the rugged dielectric structure of the crush cell layer. The SFG spectra as adoi:10.4236/jbnb.2012.322035 fatcat:2n236yydyzcxbbub7pogj3mggm