Development of a Procedure for the Determination of Three Selected Pharmaceuticals in Human Urine by Means of HPLC

Anna Gnida, Dorota Marciocha, Jolanta Turek-Szytow
2017 Engineering and Protection of Environment  
Opracowanie procedury oznaczania trzech związków z grupy farmaceutyków w próbkach moczu ludzkiego z wykorzystaniem procedury opartej na zastosowaniu techniki HPLC Development of a Procedure for the Determination of Three Selected Pharmaceuticals in Human Urine by Means of HPLC The aim of the study was to examine the possibility of determination and optimization of methodology for analysis of three selected drugs: trimethoprim, sulfamethoxazole and diclofenac in urine using high-performance
more » ... gh-performance liquid chromatography, without extraction of the pharmaceuticals to the solid phase. The chromatographic separation conditions were determined using a gradient elution system and mobile phase, which was a mixture of acetonitrile and a phosphate buffer, allowing detection of trimethoprim, sulfamethoxazole and diclofenac from 260, 210 and 310 µg/L respectively, and simultaneous determination of these analytes in the presence of urine components.
doi:10.17512/ios.2017.4.1 fatcat:wwi5674nfrf7rkbumwqap3iyji