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This study was designed to test the hypothesis that there is heterogeneous expression of contractile and structural proteins between the smooth muscle cells (SMCs) in the inner and outer circular muscle (ICM and OCM) layers of the ileum. Immunohistochemical staining and quantitation of fresh frozen sections of the dog ileum was performed using protein specific antibodies. Smooth muscle (SM) SMA myosin heavy chain (MHC), αand γ -SM actin, and vinculin all show greater expression in the ICMdoi:10.1540/jsmr.45.217 pmid:19907120 fatcat:sbbgeo75xvcrppiblyxuy7qaha