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Pertaining to reported high prevalence of brucellosis in Pakistan, district Layyah on account of having thick sheep population was selected for this study. A total of 384 sheep blood samples were collected randomly from different selected private herds in the district, and tested through Modified Rose Bengal (mRB) test and Indirect Enzyme Linked Immunosorbent Assay (iELISA) for the serological analysis against the Brucella antibodies. Positive samples from these two tests were further subjecteddoaj:d8c26f28b8a548f6bf9401268ff59f3e fatcat:6ajpdu5a6bfhrk4wqymcjng3rm