High-Throughput Array Production Using Precision Glass Syringes

J. Shieh, C. To, J. Carramao, N. Nishimura, Y. Maruta, Y. Hashimoto, D. Wright, H.-c. Wu, A. Azarani
2002 BioTechniques  
The advantages of using 1, 96, or 384 precision glass syringes in automated highthroughput microdispensers in creating highly uniform and reproducible DNA, protein, and organic compound array filters and slides are described. Using the Hydra ® Microdispenser and Tango ™ Liquid Handling system, 0.1-5 ng (in 50-300 nL) PCRamplified, human cancer-related genes and housekeeping genes were spotted onto nylon membranes and coated slides. Protein solutions of 50 µ g/mL to 1 mg/mL were spotted onto
more » ... re spotted onto coated slides or onto MaxiSorp ™ 96-well plates. Up to 6144 spots/membrane and up to 1000 spots/slide were printed. The size of the spots created by glass syringes was uniform and repro -ducible (precision variation of less than 5%) from spot to spot and membrane to membrane. Using a Tango 384 system, a total of ten 6144-spot filters can be produced in approximately 25 min, translating into a spotting speed of 2.5 min/membrane.
doi:10.2144/02326mt07 pmid:12074167 fatcat:sqnw26um4jhxtjhajlerlmrcce