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Spent mushroom substrate (SMS), a renewable bio-waste from the mushroom-growing industry, was used as an adsorbent to remove methylene blue (MB) from aqueous solution. SMS was characterized using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Adsorption experiments with the SMS adsorbent were performed based on various parameters, such as adsorbent dose, initial MB dye concentration, initial pH, contact time, and temperature. The Langmuir, Freundlich, anddoi:10.15376/biores.8.3.4722-4734 fatcat:3e5xpkuxafcx7jd7zqautwimnm