Isolation of Serotype 2 Marek's Disease Virus from a Cell Line of Avian Lymphoid Leukosis

Hiroshi HIHARA, Kunitoshi IMAI, Kenji TSUKAMOTO, Kikuyasu NAKAMURA
1998 Journal of Veterinary Medical Science  
To determine a serotype of Marek's disease virus (MDV) persistently infected in a lympohoid leukosis (LL)-cell line, LSCC-BK3, clone A (BK3A), and to examine the pathogenicity of the virus, an attempt was made to isolate MDV from culture fluid of the LLcell line, using chick embryo fibroblast cultures resistant to infection with subgroup A avian leukosis virus (ALV) to eliminate subgroup A ALV. The MDV isolate, serologically identified as serotype 2 MDV and designated as the 2H strain, was free
more » ... 2H strain, was free from ALV and reticuloendotheliosis virus. A serotype 2-specific antigen of MDV was detected in 44% of BK3A, but antigens of serotypes 1 and 3 were not detected in this cell line, by the indirect immunofluorescent antibody test using serotype-specific monoclonal antibodies. MDV antigens were undetectable in LSCC-BK3, clone 2C; both cell lines were established from the same chicken. Neither clinical signs nor macroscopic lesions were observed in any of 19 chicks inoculated with the 2H strain. Three out of 19 chicks histopathologically examined had no lesions. These results suggest that serotype 2 MDV can persist in B cells transformed by ALV without cytopathic effect at a high rate, and the isolate may become a candidate for MD vaccine strains. -KEY WORDS: lymphoid leukosis cell line, Marek's disease virus, serotype 2 Marek's disease virus.
doi:10.1292/jvms.60.143 pmid:9524935 fatcat:nkwtqmxn4re6fcivrwmzkakiru