Tumoral Necrosis Factor (TNF) mRNA gene expression in relation to Vascular Endothelial Growth Factor (VEGF) and chemokines mRNA gene expression in tumoral microenvironment of endometrial cancer versus normal endometrium: Preliminary results

Giannice Raffaella, Erreni Marco, Gaetano Valenti, Yael Hants, Hooman Soleymani-Majid, Riccardo Garruto-Campanile, Mauro Felline, Paolo Guarnerio, Allavena Paola, Tozzi Roberto
2018 Cancer Reports and Reviews  
Tumor microenvironment inflammatory cells play a major role in cancer progression. Tumour Necrosis Factor (TNF) induces apoptosis in a variety of cancer cells playing a role in cancer growth and metastasis. Some chemokines and growth factors are related to TNF in many tumours. In this preliminary study we compared the TNF, VEGF, CXCL8, CXCL12, CXCL11 mRNA gene expression in normal endometrium (NE) versus endometrial cancer (EC) microenvironment. Materials and methods: Fresh samples of EC tissue
more » ... amples of EC tissue and NE were extracted from 15 patients with FIGO stage I-III undergoing primary surgery. Some of the tissue was sent for histology and part of it was treated with RNA later and stored at -80℃. RNA was reverse-transcribed, treated with DNase I, quantified and reversetranscribed into cDNA using random primers. A real-time quantitative PCR determined the relative cDNA levels of genes in each sample. Results: We collected tissue samples from EC and from NE in 15 patients with endometrial cancer FIGO stage I-IIIC. Four patients dropped out. In the comparison between NE versus EC, we found down-regulation of TNF mRNA in 73%, VEGF mRNA up-regulation in 54%, CXCL12 down-regulation in 91% of samples, down-regulation of CXCL11 of 54% and CXCL8 up-regulation in 64% of samples. Expression of TNF was significative directly related to the expression of CXCL12 (P=0.01) and of CXCL8 (P=0.05). Conclusion: Directly statistically significant correlation found among downregulation of TNF, CXCL12 and CXCL8, seem to suggest a reduced ability of the host immune response against the cancer invasiveness and growth of the tumoral microenvironment. Unexpectedly, no significant correlation was found between VEGF and CXCL12, CXCL8 and CXCL11.We will expect from a further analysis on a greater sample size the definitive and more significant results.
doi:10.15761/crr.1000174 fatcat:je776wus2vhazjgflqanitxof4