Assessment of the FilmArray® multiplex PCR system and associated meningitis/encephalitis panel in the diagnostic service of a tertiary hospital

Amanda Mostyn, Marie Lenihan, Donnchadh O'Sullivan, Sara Woods, Maureen O'Hara, James Powell, Lorraine Power, Nuala H. O'Connell, Colum P. Dunne
2020 Infection Prevention in Practice  
Keywords: Multiplex PCR Meningitis/encephalitis Diagnosis Reduced time Improved patient outcome Economic benefit S U M M A R Y Rapid and accurate diagnosis of meningitis/encephalitis (M/E) is essential for successful patient outcomes. The FilmArrayÒ meningitis/encephalitis Panel (MEP) is a multiplexed PCR test for simultaneous, rapid detection of pathogens directly from cerebrospinal fluid (CSF) samples. 94 prospectively collected CSF specimens from patients with clinical suspicion of infective
more » ... picion of infective M/E underwent testing for 14 pathogens simultaneously, including Escherichia coli, Haemophilus influenzae, Neisseria meningitidis, and Varicella zoster. MEP demonstrated 95% agreement with current PCR methods, resulting in 16 diagnosed cases of M/E. Typically, the FilmArrayÒ MEP results were delivered within approximately one hour, contrasting with current practices taking up to 5.6 days. Given the significant morbidity and mortality associated with delayed diagnosis of central nervous system infections, the FilmArrayÒ MEP is a useful addition to the diagnostic capabilities of a clinical microbiology department.
doi:10.1016/j.infpip.2020.100042 fatcat:6xyuyywkt5c6nab6zhme5atbjy