Sub-acute toxicity assessment of Sapium ellipticum (Hochst) Pax ethanol leaf extract in Wistar rats

Osasenaga Ighodaro, Abiola Adeosun, Basirat Adeoye, Oluwafemi Soetan
2017 Journal of Coastal Life Medicine  
Objective: To determine the toxicity of Sapium ellipticum (S. ellipticum) ethanol leaf extract in animal model. Methods: Three groups (I, II and III, n = 10) of Wistar rats were respectively given 0.5 mL corn oil, 400 and 800 mg/kg BW of S. ellipticum extract, twice daily, for a period of 14 days. Lethal dose (LD 50 ) was determined and morbidity was assessed via repeated dose administration of the extract. Results: S. ellipticum at the doses employed did not cause any significant changes in
more » ... icant changes in the serum concentrations of ALT, AST and ALP compared to the control group. The level of total serum cholesterol was significantly (P ˂ 0.05) and dose-dependently reduced in the extracttreated animals by 13.5% and 16.0% compared to the control animals. Total plasma protein concentration and body weight were respectively increased by 8.7% and 13.7% at 800 mg dosage of the extract. The intraperitoneal (i.p.) and intramuscular (i.m.) LD 50 values of S. ellipticum leaf extract were determined as 979.80 and 1 341.60 mg/kg BW respectively while the oral (p.o.) LD 50 was estimated to be greater than 45 000 mg/kg BW. Conclusions: The observations noted in this study apparently validate the use of S. ellipticum ethanol leaf extract in folklore medicine in terms of herbal safety.
doi:10.12980/jclm.5.2017j7-80 fatcat:zpo44uz5izawhi3zoiaj75vire