Hepatocyte growth factor-loaded ultrasound contrast agent for restoration of injured facial nerves in Sprague Dawley (SD) rats

Ya-ning HAO
2012 Journal of Medicinal Plants Research  
The purpose of the study was to explore the regeneration effect of the ultrasound-targeted microbubble destruction-mediated hepatocyte growth factor (HGF) gene system on facial nerve injury in rats. Forty (40) rats were randomly divided into 4 groups after the models of facial nerve injury were established: A, phosphate buffered saline (PBS) group; B, HGF and microbubble (HGF + MB) group; C, HGF and ultrasound (HGF + US) group; D, HGF + ultrasound + microbubble (HGF + MB/US) group. Rats were
more » ... rificed, and facial nerves were enucleated at 7, 14, 21 and 28 days after treatment. Gene and protein levels of HGF were detected by quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) and western blot, respectively. In the rat facial nerve injury model, HGF gene and protein expression were significantly higher in the injured facial nerves of the HGF + MB/US group compared to those of the other groups (P < 0.05). Ultrasound-targeted microbubble destruction effectively transfects the HGF gene into target tissues and has a significant effect on an injured facial nerve, thus, providing a new strategy for gene therapy in facial nerve injury.
doi:10.5897/jmpr12.038 fatcat:s7dzau36ozfrpineqk6wcxsq4u