Impact of the use of Ethanolic Extract of Propolis, Flavonoid and Non-Flavonoid Propolis for Direct Pulp Capping in Collagen Type I Density

Ardo Sabir, Latief Mooduto, Cahyono Kaelan, Sherly Horax
2017 Brazilian Journal of Oral Sciences  
Aim: To analysis collagen type I density on inflamed rat dental pulp after capping with propolis. Methods: Flavonoid and non-flavonoid substances were purified from propolis. Eighty male rats were divided into five groups, each group consisting of 16 rats. As a negative control (group I), rats were not conducted any treatment. A class I cavity was prepared on the occlusal surface of right maxillary first molar. Dental pulp was exposed and allowed in oral environment for 60 minutes, then dental
more » ... nutes, then dental pulp capping with ethanolic extract of propolis (group II), flavonoid propolis (group III), non-flavonoid propolis (group IV), or calcium hydroxide as positive control (group V). Rats were sacrificed at 6 hours, 2, 4 or 7 days, biopsy samples were obtained, stained and viewed by light microscope. Data was statistically analysis using Freidman and Kruskal-Wallis tests. Results: Except in group I, collagen type I density was increased in group II, III, and V with the longer of observation time periods. However, in group IV, collagen type I density increased only on day 7. No statistically significant differences of collagen type I density among the groups for each time period were found. Conclusions: Propolis and flavonoid propolis may increase collagen density on inflamed rat dental pulp.
doi:10.20396/bjos.v15i4.8650037 fatcat:wp5k6wkwwzge7adi6gg5kbk6be