Biochemical Studies on Carbohydrates

Tamazi Satoh
1949 Tohoku journal of experimental medicine  
The stomachs and small and large intestines of human cadavars, which had been examined in the Institute of Forensic Medicine and the Patho anatomical Department, were taken as materials. Mucosa was separated and after removing from mucus, extracted with water. The initial crude products were obtained according to the procedure of Sibuyai) and purified at first by the aid of glacial acetic acid and afterwards, the carbohydrate from AB stomachs excluded, with cupric hydroxide. In the exceptional
more » ... ase the substance was isolated by fractioning by means of trichloracetic acid. Of the preparations from the various sources above, those from the stomachs are dealt with in the present account. All of the carbohydrates obtained from A, B and O stomachs were found to resemble the correspond ing carbohydrate (A group specific) from hog stomachs1) in composition,2) but as regards the optical rotation even the A of them did behave dissimilar to this, which is entirely devoid of the rotatory power.3) The B and O carbohydrates agreed with each other in the latter respect, diverging from the A. It looks significant that the B carbohydrate had much less serological activity than the A. The yield of the AB carbohydrate was too small for quantitative analysis, but by analogy its composition is assumed similar to the above carbohydrates, particulary if it is a mixture of the A and B. From the initial crude product from the AB stomach mucosa was also separated a glycidamin, that again was inhibitory to isoagglutination of erythrocytes, though in a low grade. The corresponding glycidamin from hog stomachs will be referred to in the experimental part. 275
doi:10.1620/tjem.51.275 fatcat:4ylsqvz33vbbtd5xjuggyd3gfa