Zymosan Promotes Proliferation, Candida albicans Adhesion and IL-1β Production of Oral Squamous Cell Carcinoma In Vitro [post]

XU CHEN, Qingqiong Luo, Jieying Ding, Meng Yang, Ruiyang Zhang, Fuxiang Chen
2020 unpublished
Oral squamous cell carcinoma (OSCC) is the most common type of head and neck squamous cell carcinoma (HNSCC), and the effect of zymosan, a component of yeast cell wall, on oral cancer remains unclear. Cell proliferation assay was performed with CCK-8 to evaluate the effect of zymosan on proliferation of OSCC cell lines including WSU-HN4, WSU-HN6 and CAL-27, and potential mechanism was explored by quantitative real-time PCR, immunofluorescence assay and western blot. Cell adhesion assay was
more » ... sion assay was conducted to determine the adhesion of Candida albicans to OSCC cells, and related gene expression and protein was detected. Also, the proinflammatory cytokines including IL-6, IL-8, TNF-α and IL-1β from OSCC cells were determined by ELISA. In current study, zymosan (ZYM) promotes proliferation of OSCC cell lines including WSU-HN4, WSU-HN6 and CAL-27 by CCK-8 assay via TLR2/MyD88 pathway. By cell adhesion assay, the number of Candida albicans per oil field significantly increased in ZYM-treated OSCC cells compared to controls. When treated with zymosan, OSCC cells secreted significantly more pro-inflammatory cytokine IL-1β, which could enhance inflammation in oral cancer microenvironment. Zymosan from fungi cell wall promotes proliferation, Candida albicans adhesion and IL-1β production of oral squamous cell carcinoma by in-vitro experiments.
doi:10.21203/rs.3.rs-25238/v1 fatcat:a6abyaeirzfarmjoua3ondx4ja