Nanocomposite Bienzymatic Sensor for Monitoring Xanthine in Wound Diagnostics

Sohini RoyChoudhury, Yogeswaran Umasankar, Pulak Bhushan, Penelope A. Hirt, Flor E. MacQuhae, Luis J. Borda, Hadar A. Lev-Tov, Robert Kirsner, Shekhar Bhansali
2019 Journal of the Electrochemical Society  
This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM −1 cm −2 the biosensor offers
more » ... e biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.
doi:10.1149/2.0401909jes fatcat:vfjxubwocfftdbbfqmyv7dvjsi