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A simple and accurate HPLC method was developed for the determination of pheophytinatoiron( III) chloride ( PheoFeCl ) prepared by the replacement of magnesium( I I ) in chlorophyll [pheophytinatomagnesium( II )] with iron( III). The good separation of PheoFeCl was obtained by using a stationary phase of C18 chemically bonded to silica gel and a mobile phase of acetone-acetonitrile (50:50, v/v). A tailing of the PheoFeCl peak due to the dissociation of ion pairs was improved by addition ofdoi:10.2116/analsci.3.353 fatcat:fohp6us4ivgbnp6rfp4dk723i4