Yellow Fever Vaccine: Direct Challenge of Monkeys Given Graded Doses of 17D Vaccine

Richard A. Mason, Nicola M. Tauraso, Richard O. Spertzel, Robert K. Ginn
1973 Applied microbiology  
In rhesus monkeys a wide dosage range of 17D yellow fever (YF) vaccine extending to a level even below that recommended for vaccination of man elicited an immune response providing solid protection to challenge with virulent YF virus. Forty-three of 45 monkeys vaccinated with 10283 or greater weanling mouse mean lethal doses of 17D vaccine were resistant to challenge 20 weeks later with virulent Asibi strain YF virus. Monkeys given graded doses of lesser amounts of vaccine were progressively
more » ... e susceptible to challenge. With a vaccine dose > 10283 weanling mouse mean lethal doses, plaque neutralization (PN) seroconversion rates were 90% or greater, whereas hemagglutination-inhibiting (HI) and complement-fixing (CF) seroconversion rates were unrelated to vaccine dosage and were generally in the range of 20 to 80%. Ninetysix percent (51 of 54) of immune monkeys had PN titers >0.7 log10 (fivefold) neutralization index as compared to approximately 55 to 65% who showed HI or CF titers >2 log, (fourfold) neutralization index. After challenge with Asibi strain YF virus, antibody titers of all three tests increased equally. In rhesus monkeys PN antibody titers were well correlated with YF immunity, whereas HI and CF antibody titers were not.
doi:10.1128/am.25.4.539-544.1973 fatcat:gc6c7ke2ibgw3fpbc73m6psvfy