Functional Study of CLC-1 Mutants Expressed in Xenopus Oocytes Reveals that a C-terminal Region Thr891-Ser892-Thr893 is Responsible for the Effects of Protein Kinase C Activator

Kuang-Ming Hsiao, Ren-Yu Huang, Pei-Hua Tang, Min-Jon Lin
2010 Cellular Physiology and Biochemistry  
ClC-1 plays an important part in the maintenance of membrane potential in the mammalian skeletal muscle. To investigate the phosphorylation sites responsible for the effect of PKC (protein kinase C) activator, we constructed 21 different ClC-1 mutants with mutations at predicted phosphorylation sites for PKC. The functional experiments were performed on both wildtype and mutant proteins (17 point mutants and 4 double mutants) expressed in Xenopus oocytes with two-electrode voltage-clamp
more » ... g. PMA (12myristate 13-acetate), a PKC activator, caused a right shift of half-maximum activation potential (V 1/2 ) significantly in the wild-type (from -42.9±4.4 to -13.7±1.7 mV; n = 8, P < 0.05) and most of the single mutants except the S892P (from -39.5±4.5 to -35.7±5.7 mV; n = 6) and S892D (from -10.2±4.9 to -9.6±3.5 mV; n = 4). S892D, a mutant mimicking PKCmediated phosphorylation at position 892, can also mimic the effect of wild-type treated with PMA in V 1/2 value (-10.2±4.9 mV vs -13.7±1.7 mV, n = 4 -8). However, S892A still had a significant response to PMA indicating that other sites responsible for PMA might exist. Thus double mutants are generated for the following analysis. The V 1/2 of double mutants, T891A/S892A, S892A/T893A and T891A/T893A, show no significant difference between before and after PMA treatment. We hypothesize that this structural modification results in the observed alteration of the gating properties of ClC-1 by PMA. In summary, our observations show that a C-terminal region Thr891-Ser892-Thr893, at least in part, responsible for the effect of PMA on ClC-1.
doi:10.1159/000315088 pmid:20511714 fatcat:pxxxxs4q2rdedjwfyg5urn5hzq