2N1548 Preparation method of frozen-hydrated biological samples under controlled humidity for coherent X-ray diffraction microscopy(Bioimaging 2,The 48th Annual Meeting of the Biophysical Society of Japan)
2N1548 コヒーレントX線回折顕微鏡法実験の為の湿度制御下生体粒子凍結水和試料作製装置の開発(バイオイメージング2,第49回日本生物物理学会年会)

Yuki Takayama, Masayoshi Nakasako
2011 Seibutsu Butsuri  
TheBiophysicalSociety of Japan General IncorporatedAssociation resolution, we have been developing high Tcsolution MRI, "MR Microscope". As a model system, we used mutant medaka, which lackcd tumor suppressor gene, p53, and died without any rnacroscepic tumors at their early stage of ]ife, We improved our gradient system to have Iarger linear iegion and te suppress eddy current and vibration effects. In order to prevent the autolysis during our imaging time (-20 h), we applied chemical fixation
more » ... to the medaka. With the impToved MR Microscope, we thrcc dimensionally visuaiized the fixed wild type and mutant medaka at (20 pm)' voxel. In those irnascs, wc found that the mutant medaka had sma]ler liver, intestine, spleen and gcnitaT organ than the
doi:10.2142/biophys.51.s100_5 fatcat:5zf4rz7lzbfdld4hrccn4mt5zq