Secretion and Assembly of Type IV and VI Collagens Depend on Glycosylation of Hydroxylysines

Laura Sipilä, Heli Ruotsalainen, Raija Sormunen, Naomi L. Baker, Shireen R. Lamandé, Miia Vapola, Chunguang Wang, Yoshikazu Sado, Attila Aszodi, Raili Myllylä
2007 Journal of Biological Chemistry  
Most lysines in type IV and VI collagens are hydroxylated and glycosylated, but the functions of these unique galactosylhydroxylysyl and glucosylgalactosylhydroxylysyl residues are poorly understood. The formation of glycosylated hydroxylysines is catalyzed by multifunctional lysyl hydroxylase 3 (LH3) in vivo, and we have used LH3-manipulated mice and cells as models to study the function of these carbohydrates. These hydroxylysine-linked carbohydrates were shown recently to be indispensable
more » ... the formation of basement membranes (Ruotsalainen, H.
doi:10.1074/jbc.m704198200 pmid:17873278 fatcat:vujhuk2fnrf5dnpi77xetd7u3i